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In all the samples, 60 75% of the reads were categorized as mapped to the bovine reference sequence (Table 1).Table 1: Total reads and mapped reads obtained in each sample analyzedFull size tableRPKM values13 were used to establish the total number of genes expressed in the MGT, SC, LCMEC, MFG and mMEC transcriptome. In this context, approximately 90% of the total annotated genes in the UMD3.1 bovine genome assembly were expressed in the samples under study (24,616 genes out of a total of 27,368).Correlations between RPKM expression levels for the MGT, SC, LCMEC, MFG and mMEC transcriptomesThe different sampling methods were compared by calculating a Pearson correlation between the RPKM expression levels of the 24,616 expressed genes in samples of MGT, SC, LCMEC, MFG and mMEC. Our results indicate that gene expression is highly correlated among LCMEC, MGT, SC and MFG.
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